Interferometric Reflectance Imaging Sensor, IRIS – builds on strong lineage of basic and applied research at Boston University and offers high-sensitivity and quantitative detection through an approach defying the conventional wisdom. Instead of enhancing the signal through optical resonances, we exploit the power of signal averaging in shot noise limited operation to achieve virtually unlimited sensitivity in a simple interferometric platform. IRIS technique also overcomes the Achille’s Heel of label free detection: the bulk effect – the background signal due to concentration and temperature variations in the analyte solution.
Real-time visualization of the microarray on the sensor surface provide intuitive monitoring of binding experiments.
Multiplexed binding curves are extracted from the images acquired in real-time. Antibodies with unique binding characteristics for dengue NS1 are shown here (*). Rapid determination of the serotype specificity for each antibody will aid in the creation of serotype specific immunoassays.
IRIS platform offers
compact instrument with 12” X 18” footprint including all fluidics.
IRIS is configured to have four Eppendorf tubes for samples and four 250ml bottles for wash, buffer and waste. The minimum sample volume is 100 µl and can be further reduced.
Automatic fluid handling allows for collecting and saving a sample. Flowrate can be adjusted across a large range using a syringe pump.
Custom electronic controller is integrated with an on-board computer for image acquisition and processing. User interface is platform independent and can be accessed via a web browser on local WiFi network.
Compatible with handheld devices.
Run experiments using your iPad.
Check the status without leaving your desk.
Intuitive graphic user interface to plan experiments.
Automatic spot finding compatible with .gal files.
User configurable binding experiments.
Real-time image analysis and curve generation.
We have quantitatively calibrated the interferometric sensing to the surface-bound concentrations and masses of adsorbed layers of nucleic acids and proteins allowing us to calibrate the measured height to the absolute density of surface-bound molecules. IRIS has a linear dynamic range of nearly 4 orders of magnitude, which is comparable to standard SPR and considerably better than multiplexed alternative (SPRi) (*).